Phoenix Benchtop Liquid Chromatography System

What Is the Phoenix Chromatography System?

With its advanced technology, the Phoenix Chromatography System offers improved performance and increased productivity. It can utilize state-of-the-art detectors and sensors, ensuring accurate and precise results.

The system also features an intuitive user interface, making it easy to operate and navigate. Its compact design allows for space-saving installation, perfect for laboratories with limited space.

Render of the Phoenix Chromatography System

Proudly Made in the USA

Features and Benefits

  • Versatility and Flexibility grant you complete control over the amount of pumps, column diameters, type of chromatography, and measurement instruments.
  • Proprietary Fluidics Assembly enable the ability to create a variety of products using different styles of chromatography on the same system
  • Benchtop/ Benchscale lets it easily fit in any laboratory, with small liquid volumes.
  • Custom Designed Columns allow for seamless transition to commercial scale operations with the same parameters
  • Integrated Software is easy to use and shows any system faults and when they occur. Anyone can create a process and run in minutes.
  • Increase Steady State Time with reduced process optimation time to achieve desired results faster.


  • Multiple applications
  • Excellent in small molecule separation
  • Highly chemically resistant (Peek, Stainless steel, PCTFE options)
  • Most versatile system on the market
  • Comes with ProSep’s knowledge and experience to create a scalable process
  • Comes in 100 or 300 ml/min systems


  • Add and use any monitoring instrument you wish, Ex. pH, Conductivity, RI detector, UV detector, Temp sensor, Pressure sensor and more.
  • Customize the size, material, and amount of the columns.
  • Multiple cuts of product or waste streams
  • Amount of inputs and outputs

Transforming the Approach to Chromatography

The Phoenix Benchtop liquid chromatography system has completely revolutionized our business. The system allowed us to create several new products and produce anywhere from grams to kilograms of product per day. The laboratory footprint is rather small for everything the system can accomplish. This is the only SMB system we have ever used that gives complete freedom on instrumentation and styles of separation. With the help of ProSep we were able to quickly optimize each process and know that it will scale commercially. Our team is actively working with ProSep’s engineers to design a commercial system based on our successful experiments. We can genuinely say our business would not be where it is today without the phoenix system and the help from the  ProSep team.

- Gilbert Fregozo, Operations Manager at BOSS LLC


Simulated moving bed chromatography (SMBC) is a powerful approach to chromatographic fractionation and has been well established for industrial scale production, including several top-selling pharmaceutical ingredients.

SMBC emulates countercurrent separation where the mobile phase flows in the opposite direction of the stationary phase. The stationary phase is represented by individual columns connected in series, and the mobile phase by inlet streams of Feed and Desorbent and outlet streams of Raffinate and Extract. Valves between the columns are systematically switched open or closed at timed intervals (switch time) to introduce the inlet streams and withdraw the outlet streams between the separation zones, simulating countercurrent movement of the columns. 

Separation occurs due to the differential interaction of the Feed mixture components for the column media. Components that interact more strongly with the column media are carried into the Extract, whereas weaker-interacting components move into the Raffinate. By adjusting the stream flow rates, the switch time, and the Desorbent composition, a cycle is established in which Feed and Desorbent are continuously added and highly purified products are continuously recovered.

Applications of SMBC

Pharmaceuticals and Fine Chemicals Separation

SMBC chromatography is extensively used in the pharmaceutical industry for the purification of chiral compounds. The separation of enantiomers is crucial because they can have significantly different therapeutic effects and metabolic pathways. SMBC allows for the efficient and cost-effective production of pure enantiomers of drugs, making it a key technology for producing active pharmaceutical ingredients (APIs) with high purity.

Sugar Industry

SMBC chromatography is applied in the sugar industry for the separation and purification of sugars, such as the separation of fructose and glucose. This application is particularly relevant in the production of high-fructose corn syrup (HFCS), where SMB technology allows for the efficient and selective separation of these two sugars, enabling the production of HFCS with desired fructose content.

Petrochemical Industry

In the petrochemical sector, SMBC is employed for the separation of xylene isomers. Xylenes are important chemical feedstocks used in the manufacture of plastics, synthetic fibers, and other petrochemical products. SMBC chromatography facilitates the separation of xylene isomers (ortho-, meta-, and para-xylene) on a large scale, which is essential for the efficient production of these valuable chemicals.

Biofuels and Bioproducts

SMBC chromatography is also used in the production of biofuels and bioproducts, such as the purification of biodiesel, bioethanol, and other bio-based chemicals. The technology can be applied to separate and purify fatty acid methyl esters (FAMEs) in biodiesel production or to purify ethanol from fermentation broths in bioethanol production. This application highlights the versatility of SMBC in contributing to the sustainability and efficiency of biofuel production processes.

Reverse-Phase Chromatography

Reverse-Phase Chromatography, a pivotal technique in chemistry, involves the separation of compounds based on their polarity. This method operates on the principle of hydrophobic interactions between the molecules in the sample and the stationary phase.

The stationary phase is typically non-polar or hydrophobic, which in turn interacts with the polar mobile phase. The compounds to be separated are forced to compete for solubility in these two phases, leading to their separation. The more polar the compound, the faster it moves through the system, exiting first, while less polar compounds interact more with the stationary phase, exiting later.

Applications Reverse-Phase Chromatography

Pharmaceutical and Biomedical Purification

RPC is critically important in the pharmaceutical industry for the analysis and purification of drugs and their metabolites. It is used for the quality control of pharmaceutical compounds, determining the purity of drug products, and purifying drug samples for the presence of impurities or degradation products. Additionally, in biomedical research, RPC aids in the detection and quantification of biomolecules, such as peptides, proteins, and nucleotides, within biological matrices.

Proteomics and Peptide Isolation

In the field of proteomics, RPC plays a central role in the separation and identification of proteins and peptides from complex biological samples. It is essential for characterizing the proteome of cells, tissues, or organisms and for studying protein-protein interactions, post-translational modifications, and protein structure and function. RPC's high resolution and compatibility with mass spectrometry make it a cornerstone technique in proteomic isolation.

Food and Beverage Industry

RPC is employed to refine and control the quality of food and beverages. It is used to identify and quantify vitamins, additives, flavors, and contaminants in various food matrices. This application ensures the safety and compliance of food products with regulatory standards, helps in the authentication of food by detecting adulteration, and aids in the analysis of natural compounds in food, such as polyphenols and antioxidants.

Environmental Refinement

The technique is also crucial in environmental monitoring, where it is used to detect, identify, and quantify pollutants in environmental samples, including water, soil, and air. RPC is particularly effective for refining organic compounds, such as pesticides, herbicides, pharmaceuticals, and industrial chemicals, contributing to the assessment of environmental pollution and the evaluation of water quality.


Sequential Simulated Moving Bed (SSMB) chromatography utilizes the principle of counter-current flow to separate complex mixtures into their individual components.

This technique substantially improves product yield and purity, reducing costs and time compared to traditional batch chromatography. SSMB achieves this by continuously feeding the mixture into multiple columns filled with stationary phase material, while eluent flows in the opposite direction.

The desired components adsorb onto the stationary phase, allowing undesired components to be washed away. The process is then sequentially repeated, ensuring a continuous separation process.

Applications of SSMB

Pharmaceuticals and Fine Chemicals

SSMB is widely used in the pharmaceutical industry for the purification of chiral drugs, where the separation of enantiomers is crucial due to their different biological activities. It is also employed in the production of high-purity fine chemicals, where the separation of closely related compounds or isomers is necessary. SSMB offers high purity and yield, which are essential for these applications.

Sugars and Sweeteners

SSMB plays a significant role in the food industry, particularly in the separation and purification of sugars and sweeteners. It is used to separate fructose and glucose mixtures, which are common in corn syrup production. The technology allows for a high degree of separation efficiency, crucial for producing high-fructose corn syrup and other sweeteners with specific compositions.

Petrochemicals and Fuels

In the petrochemical sector, SSMB technology is applied to separate xylene isomers, an important process for producing plastics and synthetic fibers. It can also be used for purifying biofuels and separating components in crude oil, enhancing the efficiency of fuel production processes by improving the purity and quality of the final products.

Amino Acids and Peptides

The separation and purification of amino acids, peptides, and other biomolecules are critical for nutritional supplements, pharmaceuticals, and research applications. SSMB technology facilitates the separation of these compounds based on their charge, size, and hydrophobicity differences, providing a highly efficient method for obtaining pure substances necessary for these markets.

Adsorption/ Desorption

Adsorption is the process where certain molecules are attracted to the surface of a solid or liquid, binding to it and forming a thin film. This occurs due to the molecular or ionic forces between the adsorbent (the material on the surface) and the adsorbate (the substance being adsorbed).

Conversely, desorption is the reverse process, where adsorbed molecules are released from the surface.

Applications of Adsorption/Desorption

Water Treatment

The water treatment industry employs adsorption and desorption processes to remove pollutants from water, making it safe for human consumption and environmental discharge. These processes are vital for eliminating a wide range of contaminants, including organic compounds, heavy metals, and other pollutants. 

Petroleum Industry

The petroleum industry extensively uses adsorption and desorption processes for various applications, ranging from gas purification and separation to the recovery of valuable hydrocarbons. These processes are crucial in enhancing the efficiency, sustainability, and profitability of petroleum operations. 

Other Industries

The applications of adsorption and desorption are far reaching, and can be found in a multitude of industries, such as:

  • Pharmaceutical Industry
  • Food and Beverage Industry
  • Environmental Remediation
  • Mining Industry
  • Nuclear Industry
  • Gas Separation
  • Biotechnology
  • Hydrogen Production
  • Cosmetics

Batch Chromatography

Batch chromatography, a vital technique employed in various scientific fields, is a type of chromatographic method that enables the separation and purification of components within a complex mixture. It is distinguished by its process wherein the mixture is loaded onto the stationary phase, followed by the mobile phase, in a single batch.

The components separate as they interact differently with the stationary phase, allowing for precise isolation and analysis. This technique is particularly beneficial for large-scale separations, where continuous methods would be impractical or inefficient.

Applications of Batch Chromatography

Pharmaceutical Purification and Analysis

Batch chromatography is extensively used in the pharmaceutical industry for the purification of drugs, including active pharmaceutical ingredients (APIs) and intermediates. It is also employed for the analytical characterization of pharmaceutical compounds, including the identification of impurities and the determination of drug purity. This application is crucial for ensuring the safety and efficacy of pharmaceutical products.

Food and Beverage Industry

Batch chromatography is used in the food and beverage industry for the analysis and purification of food components, such as flavors, fragrances, vitamins, and natural extracts. It helps in ensuring the quality and safety of food products by detecting contaminants and adulterants and by analyzing nutritional components for regulatory compliance.

Biotechnology and Protein Purification

In the biotechnology sector, batch chromatography plays a critical role in the purification of proteins, peptides, and other biologically derived products, such as antibodies, enzymes, and vaccines. It allows for the selective separation of target biomolecules from complex mixtures, based on their specific properties, such as size, charge, and hydrophobicity. This application is essential for producing high-purity biopharmaceuticals and for research in proteomics and genomics.

Environmental Analysis

The technique is also employed in environmental monitoring to detect and quantify pollutants in water, soil, and air samples. Batch chromatography can separate and identify organic compounds, heavy metals, and other contaminants, contributing to the assessment of environmental pollution and the evaluation of remediation strategies.

Schedule a Demo Today!

If you have any questions or would like to schedule a demo, please use this form or call us at (815) 623-7630.

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Frequently Asked Questions

Learn more at our dedicated Benchtop Liquid Chromatography FAQs Page.

The ProSep Advantage


ProSep employees have been designing and building large scale chromatography and IX equipment since the 1970’s

Robust Equipment Design

As a result of ProSep’s experience, our equipment design includes safeguards to ensure that malfunctions will not occur.


Arguably the most important aspect of a liquid separation process, ProSep distribution has evolved over the years to ensure an even flow distribution.

Process Knowledge

The ProSep team has many decades of combined knowledge with respect to the chromatographic, filtration, and Ion Exchange process.

Process Design

Every piece of equipment ProSep supplies is engineered specific to the customer’s requirements.

Get In Touch

Contact Details

  • Phone: 815.623.7630

  • Address: 9251 N 2nd St, Roscoe, IL 61073 USA

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